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iridium cell id intercalator  (fluidigm)


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    Structured Review

    fluidigm iridium cell id intercalator
    Iridium Cell Id Intercalator, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iridium+intercalator/Cell-ID+Intercalator-Ir/10__1038_slash_s41590___026___02472___z-584-0-3
    Average 96 stars, based on 1486 article reviews
    iridium cell id intercalator - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Distinct spatially resolved tumor microenvironment trajectories define benefit from ramucirumab plus pembrolizumab in refractory PD-L1 + gastric cancer
    Article Snippet: After surface staining, cells were fixed with FA solution diluted in 16% formaldehyde (Thermo Fisher Scientific, MA, USA). .. After fixation, DNA was stained with an iridium intercalator (Standard BioTools Inc., Cat# 201192A) to detect nuclei and incubated at 2–8°C overnight. .. Before data acquisition on a CyTOF XT (Standard BioTools Inc, South San Francisco, CA, USA), EQ4 beads (Standard BioTools Inc, South San Francisco, CA, USA) were added to cells to ensure that the final concentration of EQ4 beads was 10%.

    Article Title: Immune cell subsets in autoimmune polyendocrine syndrome type I.
    Article Snippet: .. Following this, cells were washed and an intercalation solution—comprising 66.5% fresh 16% paraformaldehyde (PFA) (Thermo Scientific, cat. J19943.K2), 9.5% 10X Intracellular Staining Perm Wash Buffer (BioLegend, cat. 421002), and 125nM Iridium Intercalator (Standard Biotools, cat. 201192B)—was added and left to incubate at 4°C overnight. .. After a final wash, the cells were preserved in CAS plus (Standard Biotools, #201244) until acquired using the CyTOF XT instrument (Standard Biotools).

    Article Title: Distinct spatially resolved tumor microenvironment trajectories define benefit from ramucirumab plus pembrolizumab in refractory PD-L1+ gastric cancer
    Article Snippet: After surface staining, cells were fixed with FA solution diluted in 16% formaldehyde (Thermo Fisher Scientific, MA, USA). .. After fixation, DNA was stained with an iridium intercalator (Standard BioTools Inc., Cat# 201192A) to detect nuclei and incubated at 2−8°C overnight. .. Before data acquisition on a CyTOF XT (Standard BioTools Inc, South San Francisco, CA, USA), EQ4 beads (Standard BioTools Inc, South San Francisco, CA, USA) were added to cells to ensure that the final concentration of EQ4 beads was 10%.

    Article Title: Immune cell subsets in autoimmune polyendocrine syndrome type I
    Article Snippet: .. Following this, cells were washed and an intercalation solution—comprising 66.5% fresh 16% paraformaldehyde (PFA) (Thermo Scientific, cat. J19943.K2), 9.5% 10X Intracellular Staining Perm Wash Buffer (BioLegend, cat. 421002), and 125nM Iridium Intercalator (Standard Biotools, cat. 201192B)—was added and left to incubate at 4°C overnight. .. After a final wash, the cells were preserved in CAS plus (Standard Biotools, #201244) until acquired using the CyTOF XT instrument (Standard Biotools).

    Article Title: Menin inhibitor DS-1594b drives differentiation and induces synergistic lethality in combination with venetoclax in acute myeloid leukemia cells with rearranged mixed-lineage leukemia and mutated nucleophosmin-1.
    Article Snippet: .. Next, cells were washed twice with PBS and stained for 30 minutes with antibodies against intracellular markers in a final reaction volume of 50 μL, washed twice with wash buffer and once with PBS, and stained for 20 minutes in 500 μL of 1:1000 iridium intercalator (Fluidigm; cat. 201192A) diluted in 1.6% PFA in PBS. ..

    Incubation:

    Article Title: Distinct spatially resolved tumor microenvironment trajectories define benefit from ramucirumab plus pembrolizumab in refractory PD-L1 + gastric cancer
    Article Snippet: After surface staining, cells were fixed with FA solution diluted in 16% formaldehyde (Thermo Fisher Scientific, MA, USA). .. After fixation, DNA was stained with an iridium intercalator (Standard BioTools Inc., Cat# 201192A) to detect nuclei and incubated at 2–8°C overnight. .. Before data acquisition on a CyTOF XT (Standard BioTools Inc, South San Francisco, CA, USA), EQ4 beads (Standard BioTools Inc, South San Francisco, CA, USA) were added to cells to ensure that the final concentration of EQ4 beads was 10%.

    Article Title: Granulin + macrophages promote lineage plasticity in prostate cancer through paracrine signaling loops
    Article Snippet: .. Following triple washes with CSB, cells were incubated with 0.125 μM iridium intercalator in fix and perm buffer (Fluidigm) overnight at 4 [. .. Before acquisition, samples were washed and resuspended in deionized water containing 10% EQ 4 Element Beads (Fluidigm), with cell concentrations adjusted to 1×10^6 cells/ml.

    Article Title: Comprehensive and advanced T cell cluster analysis for discriminating seropositive and seronegative rheumatoid arthritis
    Article Snippet: .. Subsequently, cell samples were incubated with 125 nM iridium intercalator (Cell-ID Intercalator-Ir 125 μM, Fluidigm) in Maxpar Fix and Perm Buffer (Fluidigm) overnight at 4°C, washed with Milli-Q water, resuspended, filtered through a 35-μm nylon mesh, and prepared with EQ Four-Element Calibration Beads (Fluidigm), according to the manufacturer’s protocol. .. The samples were analyzed using a Helios mass cytometer and CyTOF System (Fluidigm).

    Article Title: Distinct spatially resolved tumor microenvironment trajectories define benefit from ramucirumab plus pembrolizumab in refractory PD-L1+ gastric cancer
    Article Snippet: After surface staining, cells were fixed with FA solution diluted in 16% formaldehyde (Thermo Fisher Scientific, MA, USA). .. After fixation, DNA was stained with an iridium intercalator (Standard BioTools Inc., Cat# 201192A) to detect nuclei and incubated at 2−8°C overnight. .. Before data acquisition on a CyTOF XT (Standard BioTools Inc, South San Francisco, CA, USA), EQ4 beads (Standard BioTools Inc, South San Francisco, CA, USA) were added to cells to ensure that the final concentration of EQ4 beads was 10%.



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